› 1fl5

X-ray diffraction
2.1Å resolution

THE UNLIGANDED GERMLINE PRECURSOR TO THE SULFIDE OXIDASE CATALYTIC ANTIBODY 28B4.

Released:
DOI: 10.2210/pdb1fl5/pdb
PDB entry 1fl5 coloured by chain, front view.
PDB entry 1fl5 coloured by chain, side view.
PDB entry 1fl5 coloured by chain, top view.
Source organisms:
Primary publication:
A comparative analysis of the immunological evolution of antibody 28B4.
Yin J, Mundorff EC, Yang PL, Wendt KU, Hanway D, Stevens RC, Schultz PG
Biochemistry 40 10764-73 (2001)
PMID: 11535051

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213003 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ANTIBODY GERMLINE PRECURSOR TO ANTIBODY 28B4 Chains: A, L
Molecule details ›
Chains: A, L
Length: 217 amino acids
Theoretical weight: 23.79 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
ANTIBODY GERMLINE PRECURSOR TO ANTIBODY 28B4 Chains: B, H
Molecule details ›
Chains: B, H
Length: 219 amino acids
Theoretical weight: 23.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P1
Unit cell:
a: 36.42Å b: 69.65Å c: 85.31Å
α: 74.56° β: 80.18° γ: 77.55°
R-values:
R R work R free
0.238 0.226 0.268
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Survey of the year 2001 commercial optical biosensor literature.
Rich et al. (2002)
4 more

6 mentions without citation

Multi-constraint computational design suggests that native sequences of germline antibody H3 loops are nearly optimal for conformational flexibility.
Babor et al. (2009)
5 more