› 1fph

X-ray diffraction
2.5Å resolution

THE INTERACTION OF THROMBIN WITH FIBRINOGEN: A STRUCTURAL BASIS FOR ITS SPECIFICITY

Released:
DOI: 10.2210/pdb1fph/pdb
PDB entry 1fph coloured by chain, front view.
PDB entry 1fph coloured by chain, side view.
PDB entry 1fph coloured by chain, top view.
Source organisms:
Primary publication:
The interaction of thrombin with fibrinogen. A structural basis for its specificity.
Stubbs MT, Oschkinat H, Mayr I, Huber R, Angliker H, Stone SR, Bode W
Eur J Biochem 206 187-95 (1992)
PMID: 1587268

Function and Biology Details

Reaction catalysed: 
Selective cleavage of Arg-|-Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133068 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Thrombin light chain Chain: L
Molecule details ›
Chain: L
Length: 36 amino acids
Theoretical weight: 4.1 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P00734 (Residues: 328-363; Coverage: 6%)
Gene name: F2
Sequence domains: Thrombin light chain
Thrombin heavy chain Chain: H
Molecule details ›
Chain: H
Length: 259 amino acids
Theoretical weight: 29.78 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P00734 (Residues: 364-622; Coverage: 43%)
Gene name: F2
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Hirudin-2B Chain: I
Molecule details ›
Chain: I
Length: 12 amino acids
Theoretical weight: 1.47 KDa
Source organism: Hirudo medicinalis
Expression system: Not provided
UniProt:
  • Canonical: P28506 (Residues: 54-65; Coverage: 19%)
Fibrinopeptide A Chain: F
Molecule details ›
Chain: F
Length: 12 amino acids
Theoretical weight: 1.08 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P02671 (Residues: 26-35; Coverage: 1%)
Gene name: FGA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P43212
Unit cell:
a: 90.34Å b: 90.34Å c: 132.34Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.166 not available
Expression system: Not provided

Quick links

Citations

16 review citations

A player of many parts: the spotlight falls on thrombin's structure.
Stubbs et al. (1993)
15 more

6 mentions without citation

Impact of structural biologists and the Protein Data Bank on small-molecule drug discovery and development.
Burley SK. (2021)
5 more