› 1fs9

X-ray diffraction
2Å resolution

CYTOCHROME C NITRITE REDUCTASE FROM WOLINELLA SUCCINOGENES-AZIDE COMPLEX

Released:
DOI: 10.2210/pdb1fs9/pdb
PDB entry 1fs9 coloured by chain, front view.
PDB entry 1fs9 coloured by chain, side view.
PDB entry 1fs9 coloured by chain, top view.
Source organism: Wolinella succinogenes
Primary publication:
Cytochrome c nitrite reductase from Wolinella succinogenes. Structure at 1.6 A resolution, inhibitor binding, and heme-packing motifs.
Einsle O, Stach P, Messerschmidt A, Simon J, Kröger A, Huber R, Kroneck PM
J Biol Chem 275 39608-16 (2000)
PMID: 10984487

Function and Biology Details

Reaction catalysed: 
NH(3) + 2 H(2)O + 6 ferricytochrome c = nitrite + 6 ferrocytochrome c + 7 H(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193377 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome c-552 Chain: A
Molecule details ›
Chain: A
Length: 507 amino acids
Theoretical weight: 57.6 KDa
Source organism: Wolinella succinogenes
UniProt:
  • Canonical: Q9S1E5 (Residues: 1-507; Coverage: 100%)
Gene names: WS0969, nrfA
Sequence domains: Cytochrome c552
Structure domains:

Ligands and Environments


Cofactor: Ligand HEC 5 x HEC
3 bound ligands:
Ligand CA 1 x CA
Ligand Y1 3 x Y1
Ligand AZI 1 x AZI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: I4122
Unit cell:
a: 119.532Å b: 119.532Å c: 186.056Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.18 0.208

Quick links

Citations

10 review citations

Enzymology and bioenergetics of respiratory nitrite ammonification.
Simon J. (2002)
9 more

1 mention without citation

Nature's nitrite-to-ammonia expressway, with no stop at dinitrogen.
Kroneck PMH. (2022)