› 1fsu

X-ray diffraction
2.5Å resolution

Crystal Structure of 4-Sulfatase (human)

Released:
DOI: 10.2210/pdb1fsu/pdb
PDB entry 1fsu coloured by chain, front view.
PDB entry 1fsu coloured by chain, side view.
PDB entry 1fsu coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of a human lysosomal sulfatase.
Bond CS, Clements PR, Ashby SJ, Collyer CA, Harrop SJ, Hopwood JJ, Guss JM
Structure 5 277-89 (1997)
PMID: 9032078

Function and Biology Details

Reaction catalysed: 
Hydrolysis of the 4-sulfate groups of the N-acetyl-D-galactosamine 4-sulfate units of chondroitin sulfate and dermatan sulfate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147503 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Arylsulfatase B Chain: A
Molecule details ›
Chain: A
Length: 492 amino acids
Theoretical weight: 55.82 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P15848 (Residues: 42-533; Coverage: 99%)
Gene name: ARSB
Sequence domains: Sulfatase
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand CA 1 x CA
Ligand CL 1 x CL
1 modified residue:
Ligand ALS 1 x ALS

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P41212
Unit cell:
a: 107Å b: 107Å c: 144.83Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.188 0.243
Expression system: Cricetulus griseus

Quick links

Citations

34 review citations

Catalytic promiscuity and the evolution of new enzymatic activities.
O'Brien et al. (1999)
33 more

28 mentions without citation

Divergence and convergence in enzyme evolution.
Galperin et al. (2012)
27 more