› 1fv2

X-ray diffraction
2.5Å resolution

The Hc fragment of tetanus toxin complexed with an analogue of its ganglioside receptor GT1B

Released:
DOI: 10.2210/pdb1fv2/pdb
PDB entry 1fv2 coloured by chain, front view.
PDB entry 1fv2 coloured by chain, side view.
PDB entry 1fv2 coloured by chain, top view.
Source organism: Clostridium tetani
Primary publication:
The crystal structure of tetanus toxin Hc fragment complexed with a synthetic GT1b analogue suggests cross-linking between ganglioside receptors and the toxin.
Fotinou C, Emsley P, Black I, Ando H, Ishida H, Kiso M, Sinha KA, Fairweather NF, Isaacs NW
J Biol Chem 276 32274-81 (2001)
PMID: 11418600

Function and Biology Details

Reaction catalysed: 
Hydrolysis of -Gln(76)-|-Phe- bond in synaptobrevin (also known as neuronal vesicle-associated membrane protein, VAMP).
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138291 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Tetanus toxin heavy chain Chain: A
Molecule details ›
Chain: A
Length: 472 amino acids
Theoretical weight: 54 KDa
Source organism: Clostridium tetani
Expression system: Escherichia coli
UniProt:
  • Canonical: P04958 (Residues: 862-1315; Coverage: 35%)
Gene names: CTC_p60, tetX
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GAL, NGA, SIA, SLB
Carbohydrate polymer
2 bound ligands:
Ligand PO4 1 x PO4
Ligand CEQ 1 x CEQ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX7.2
Spacegroup: P21
Unit cell:
a: 45.849Å b: 52.172Å c: 117.353Å
α: 90° β: 99.55° γ: 90°
R-values:
R R work R free
0.225 0.224 0.276
Expression system: Escherichia coli

Quick links

Citations

19 review citations

Identification of the major steps in botulinum toxin action.
Simpson LL. (2004)
18 more

11 mentions without citation

Tetanus Toxin Fragment C: Structure, Drug Discovery Research and Production.
Bayart et al. (2022)
10 more