› 1fva

X-ray diffraction
1.7Å resolution

CRYSTAL STRUCTURE OF BOVINE METHIONINE SULFOXIDE REDUCTASE

Released:
DOI: 10.2210/pdb1fva/pdb
PDB entry 1fva coloured by chain, front view.
PDB entry 1fva coloured by chain, side view.
PDB entry 1fva coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Structure and mechanism of peptide methionine sulfoxide reductase, an "anti-oxidation" enzyme.
Lowther WT, Brot N, Weissbach H, Matthews BW
Biochemistry 39 13307-12 (2000)
PMID: 11063566

Function and Biology Details

Reaction catalysed: 
L-methionine + thioredoxin disulfide + H(2)O = L-methionine (S)-S-oxide + thioredoxin
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156950 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitochondrial peptide methionine sulfoxide reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 217 amino acids
Theoretical weight: 24.03 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P54149 (Residues: 13-229; Coverage: 93%)
Gene name: MSRA
Sequence domains: Peptide methionine sulfoxide reductase
Structure domains: Peptide methionine sulphoxide reductase MsrA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 53.71Å b: 66.199Å c: 62.137Å
α: 90° β: 90.99° γ: 90°
R-values:
R R work R free
0.207 0.207 0.28
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Oxidative stress and protein aggregation during biological aging.
Squier TC. (2001)
17 more

5 mentions without citation

The methionine sulfoxide reduction system: selenium utilization and methionine sulfoxide reductase enzymes and their functions.
Kim HY. (2013)
4 more