› 1fvi

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF CHLORELLA VIRUS DNA LIGASE-ADENYLATE

Released:
DOI: 10.2210/pdb1fvi/pdb
PDB entry 1fvi coloured by chain, front view.
PDB entry 1fvi coloured by chain, side view.
PDB entry 1fvi coloured by chain, top view.
Source organism: Chlorella virus
Primary publication:
Crystal structure of eukaryotic DNA ligase-adenylate illuminates the mechanism of nick sensing and strand joining.
Odell M, Sriskanda V, Shuman S, Nikolov DB
Mol Cell 6 1183-93 (2000)
PMID: 11106756

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108382 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA ligase Chain: A
Molecule details ›
Chain: A
Length: 297 amino acids
Theoretical weight: 34.12 KDa
Source organism: Chlorella virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A7RCB1 (Residues: 27-322; Coverage: 92%)
Gene names: AR158_C658R, C658R
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand AMP 1 x AMP
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 48.17Å b: 58.44Å c: 101.79Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.19 0.27
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

14 review citations

Complex management: RNA editing in trypanosomes.
Stuart et al. (2005)
13 more

9 mentions without citation

DNA ligases: progress and prospects.
Shuman S. (2009)
8 more