› 1fzr

X-ray diffraction
2.1Å resolution

CRYSTAL STRUCTURE OF BACTERIOPHAGE T7 ENDONUCLEASE I

Released:
DOI: 10.2210/pdb1fzr/pdb
PDB entry 1fzr coloured by chain, front view.
PDB entry 1fzr coloured by chain, side view.
PDB entry 1fzr coloured by chain, top view.
Source organism: Escherichia phage T7
Primary publication:
Crystal structure of the Holliday junction resolving enzyme T7 endonuclease I.
Hadden JM, Convery MA, Déclais AC, Lilley DM, Phillips SE
Nat Struct Biol 8 62-7 (2001)
PMID: 11135673

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132788 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endonuclease I Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 138 amino acids
Theoretical weight: 16.07 KDa
Source organism: Escherichia phage T7
Expression system: Escherichia coli
UniProt:
  • Canonical: P00641 (Residues: 12-149; Coverage: 93%)
Gene name: 3
Sequence domains: Phage endonuclease I
Structure domains: Restriction Endonuclease

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P21212
Unit cell:
a: 123.66Å b: 135.19Å c: 61.58Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.198 0.235
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Holliday junction resolvases.
Wyatt et al. (2014)
6 more

6 mentions without citation

The PD-(D/E)XK superfamily revisited: identification of new members among proteins involved in DNA metabolism and functional predictions for domains of (hitherto) unknown function.
Kosinski et al. (2005)
5 more