› 1g0t

X-ray diffraction
2.6Å resolution

DSBC MUTANT C101S

Released:
DOI: 10.2210/pdb1g0t/pdb
PDB entry 1g0t coloured by chain, front view.
PDB entry 1g0t coloured by chain, side view.
PDB entry 1g0t coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of the protein disulfide bond isomerase, DsbC, from Escherichia coli.
McCarthy AA, Haebel PW, Törrönen A, Rybin V, Baker EN, Metcalf P
Nat Struct Biol 7 196-9 (2000)
PMID: 10700276

Function and Biology Details

Reaction catalysed: 
Catalyzes the rearrangement of -S-S- bonds in proteins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142455 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiol:disulfide interchange protein DsbC Chains: A, B
Molecule details ›
Chains: A, B
Length: 216 amino acids
Theoretical weight: 23.47 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEG6 (Residues: 21-236; Coverage: 100%)
Gene names: JW2861, b2893, dsbC, xprA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P212121
Unit cell:
a: 59.441Å b: 78.501Å c: 95.697Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.212 0.274
Expression system: Escherichia coli

Quick links

Citations

55 review citations

Recombinant protein folding and misfolding in Escherichia coli.
Baneyx et al. (2004)
54 more

2 mentions without citation

Photoreceptor phosphodiesterase (PDE6): activation and inactivation mechanisms during visual transduction in rods and cones.
Cote RH. (2021)
1 more