› 1g27

X-ray diffraction
2.1Å resolution

CRYSTAL STRUCTURE OF E.COLI POLYPEPTIDE DEFORMYLASE COMPLEXED WITH THE INHIBITOR BB-3497

Released:
DOI: 10.2210/pdb1g27/pdb
PDB entry 1g27 coloured by chain, front view.
PDB entry 1g27 coloured by chain, side view.
PDB entry 1g27 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Antibiotic activity and characterization of BB-3497, a novel peptide deformylase inhibitor.
Clements JM, Beckett RP, Brown A, Catlin G, Lobell M, Palan S, Thomas W, Whittaker M, Wood S, Salama S, Baker PJ, Rodgers HF, Barynin V, Rice DW, Hunter MG
Antimicrob Agents Chemother 45 563-70 (2001)
PMID: 11158755

Function and Biology Details

Reaction catalysed: 
Formyl-L-methionyl peptide + H(2)O = formate + methionyl peptide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141367 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptide deformylase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 168 amino acids
Theoretical weight: 19.23 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A6K3 (Residues: 2-169; Coverage: 99%)
Gene names: JW3248, b3287, def, fms
Sequence domains: Polypeptide deformylase
Structure domains: Peptide deformylase

Ligands and Environments

2 bound ligands:
Ligand NI 3 x NI
Ligand BB1 3 x BB1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2
Unit cell:
a: 143.14Å b: 64.47Å c: 85.29Å
α: 90° β: 123.11° γ: 90°
R-values:
R R work R free
0.21 0.21 0.27
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

21 review citations

Challenges of antibacterial discovery.
Silver LL. (2011)
20 more

4 mentions without citation

Targeting Metalloenzymes for Therapeutic Intervention.
Chen et al. (2019)
3 more