› 1g2i

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF A NOVEL INTRACELLULAR PROTEASE FROM PYROCOCCUS HORIKOSHII AT 2 A RESOLUTION

Released:
DOI: 10.2210/pdb1g2i/pdb
PDB entry 1g2i coloured by chain, front view.
PDB entry 1g2i coloured by chain, side view.
PDB entry 1g2i coloured by chain, top view.
Source organism: Pyrococcus horikoshii
Primary publication:
Crystal structure of an intracellular protease from Pyrococcus horikoshii at 2-A resolution.
Du X, Choi IG, Kim R, Wang W, Jancarik J, Yokota H, Kim SH
Proc. Natl. Acad. Sci. U.S.A. 97 14079-84 (2000)
PMID: 11114201

Function and Biology Details

Reaction catalysed: 
An N(6)-(1-hydroxy-2-oxopropyl)-[protein]-L-lysine + H(2)O = a [protein]-L-lysine + lactate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129858 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Deglycase PH1704 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 166 amino acids
Theoretical weight: 18.84 KDa
Source organism: Pyrococcus horikoshii
Expression system: Escherichia coli
UniProt:
  • Canonical: O59413 (Residues: 1-166; Coverage: 100%)
Gene name: PH1704
Sequence domains: DJ-1/PfpI family
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P41212
Unit cell:
a: 124.7Å b: 124.7Å c: 129Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.184 0.2
Expression system: Escherichia coli

Quick links

Citations

9 review citations

The role of cysteine oxidation in DJ-1 function and dysfunction.
Wilson MA. (2011)
8 more

11 mentions without citation

Crystal structure of an intracellular protease from Pyrococcus horikoshii at 2-A resolution.
Du et al. (2000)
10 more