› 1g5r

X-ray diffraction
2.1Å resolution

THE THREE-DIMENSIONAL STRUCTURE OF ATP:CORRINOID ADENOSYLTRANSFERASE FROM SALMONELLA TYPHIMURIUM. APO FORM

Released:
DOI: 10.2210/pdb1g5r/pdb
PDB entry 1g5r coloured by chain, front view.
PDB entry 1g5r coloured by chain, side view.
PDB entry 1g5r coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Primary publication:
Three-dimensional structure of ATP:corrinoid adenosyltransferase from Salmonella typhimurium in its free state, complexed with MgATP, or complexed with hydroxycobalamin and MgATP.
Bauer CB, Fonseca MV, Holden HM, Thoden JB, Thompson TB, Escalante-Semerena JC, Rayment I
Biochemistry 40 361-74 (2001)
PMID: 11148030

Function and Biology Details

Reaction catalysed: 
(1a) 2 cob(II)alamin + 2 [corrinoid adenosyltransferase] = 2 [corrinoid adenosyltransferase]-cob(II)alamin
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152035 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Corrinoid adenosyltransferase CobA Chain: A
Molecule details ›
Chain: A
Length: 196 amino acids
Theoretical weight: 22.08 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Salmonella enterica subsp. enterica serovar Typhimurium
UniProt:
  • Canonical: P31570 (Residues: 1-196; Coverage: 100%)
Gene names: STM1718, btuR, cobA
Sequence domains: ATP:corrinoid adenosyltransferase BtuR/CobO/CobP
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
1 modified residue:
Ligand MSE 7 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P6522
Unit cell:
a: 49.4Å b: 49.4Å c: 249.8Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 0.178 0.247
Expression system: Salmonella enterica subsp. enterica serovar Typhimurium

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Citations

3 review citations

Enzymes that catalyse SN2 reaction mechanisms.
O'Hagan et al. (2010)
2 more

3 mentions without citation

Selective prediction of interaction sites in protein structures with THEMATICS.
Wei et al. (2007)
2 more