› 1g64

X-ray diffraction
2.1Å resolution

THE THREE-DIMENSIONAL STRUCTURE OF ATP:CORRINOID ADENOSYLTRANSFERASE FROM SALMONELLA TYPHIMURIUM. COBALAMIN/ATP TERNARY COMPLEX

Released:
DOI: 10.2210/pdb1g64/pdb
PDB entry 1g64 coloured by chain, front view.
PDB entry 1g64 coloured by chain, side view.
PDB entry 1g64 coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Primary publication:
Three-dimensional structure of ATP:corrinoid adenosyltransferase from Salmonella typhimurium in its free state, complexed with MgATP, or complexed with hydroxycobalamin and MgATP.
Bauer CB, Fonseca MV, Holden HM, Thoden JB, Thompson TB, Escalante-Semerena JC, Rayment I
Biochemistry 40 361-74 (2001)
PMID: 11148030

Function and Biology Details

Reaction catalysed: 
(1a) 2 cob(II)alamin + 2 [corrinoid adenosyltransferase] = 2 [corrinoid adenosyltransferase]-cob(II)alamin
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152035 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Corrinoid adenosyltransferase CobA Chains: A, B
Molecule details ›
Chains: A, B
Length: 196 amino acids
Theoretical weight: 21.75 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Salmonella enterica subsp. enterica serovar Typhimurium
UniProt:
  • Canonical: P31570 (Residues: 1-196; Coverage: 100%)
Gene names: STM1718, btuR, cobA
Sequence domains: ATP:corrinoid adenosyltransferase BtuR/CobO/CobP
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand B12 1 x B12
Ligand ATP 2 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID, null
Spacegroup: P41212
Unit cell:
a: 80Å b: 80Å c: 142Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.206 0.288
Expression system: Salmonella enterica subsp. enterica serovar Typhimurium

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Citations

2 review citations

Enzymes that catalyse SN2 reaction mechanisms.
O'Hagan et al. (2010)
1 more

5 mentions without citation

Multiple roles of ATP:cob(I)alamin adenosyltransferases in the conversion of B12 to coenzyme B12.
Mera et al. (2010)
4 more