› 1g6x

X-ray diffraction
0.86Å resolution

ULTRA HIGH RESOLUTION STRUCTURE OF BOVINE PANCREATIC TRYPSIN INHIBITOR (BPTI) MUTANT WITH ALTERED BINDING LOOP SEQUENCE

Released:
DOI: 10.2210/pdb1g6x/pdb
PDB entry 1g6x coloured by chain, front view.
PDB entry 1g6x coloured by chain, side view.
PDB entry 1g6x coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Ultrahigh-resolution structure of a BPTI mutant.
Addlagatta A, Krzywda S, Czapinska H, Otlewski J, Jaskolski M
Acta Crystallogr D Biol Crystallogr 57 649-63 (2001)
PMID: 11320305

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-133749 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pancreatic trypsin inhibitor Chain: A
Molecule details ›
Chain: A
Length: 58 amino acids
Theoretical weight: 6.48 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00974 (Residues: 36-93; Coverage: 73%)
Sequence domains: Kunitz/Bovine pancreatic trypsin inhibitor domain
Structure domains: Pancreatic trypsin inhibitor Kunitz domain

Ligands and Environments

2 bound ligands:
Ligand SO4 8 x SO4
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P43212
Unit cell:
a: 51.891Å b: 51.891Å c: 43.035Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.107 not available 0.14
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Carbon-oxygen hydrogen bonding in biological structure and function.
Horowitz et al. (2012)
3 more

15 mentions without citation

Current Approaches in Supersecondary Structures Investigation.
Rudnev et al. (2021)
14 more