› 1g7u

X-ray diffraction
2.8Å resolution

CRYSTAL STRUCTURES OF KDO8P SYNTHASE IN ITS BINARY COMPLEX WITH SUBSTRATE PHOSPHOENOL PYRUVATE

Released:
DOI: 10.2210/pdb1g7u/pdb
PDB entry 1g7u coloured by chain, front view.
PDB entry 1g7u coloured by chain, side view.
PDB entry 1g7u coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structures of KDOP synthase in its binary complexes with the substrate phosphoenolpyruvate and with a mechanism-based inhibitor.
Asojo O, Friedman J, Adir N, Belakhov V, Shoham Y, Baasov T
Biochemistry 40 6326-34 (2001)
PMID: 11371194

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + D-arabinose 5-phosphate + H(2)O = 3-deoxy-D-manno-octulosonate 8-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-141504 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-dehydro-3-deoxyphosphooctonate aldolase Chain: A
Molecule details ›
Chain: A
Length: 284 amino acids
Theoretical weight: 30.91 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A715 (Residues: 1-284; Coverage: 100%)
Gene names: JW1206, b1215, kdsA
Sequence domains: DAHP synthetase I family
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
Ligand PEP 1 x PEP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: I23
Unit cell:
a: 117.9Å b: 117.9Å c: 117.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.228 0.281
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Kdo: a critical monosaccharide for bacteria viability.
Cipolla et al. (2010)
1 more