› 1g87

X-ray diffraction
1.6Å resolution

THE CRYSTAL STRUCTURE OF ENDOGLUCANASE 9G FROM CLOSTRIDIUM CELLULOLYTICUM

Released:
DOI: 10.2210/pdb1g87/pdb
PDB entry 1g87 coloured by chain, front view.
PDB entry 1g87 coloured by chain, side view.
PDB entry 1g87 coloured by chain, top view.
Source organism: Ruminiclostridium cellulolyticum
Primary publication:
X-Ray crystal structure of the multidomain endoglucanase Cel9G from Clostridium cellulolyticum complexed with natural and synthetic cello-oligosaccharides.
Mandelman D, Belaich A, Belaich JP, Aghajari N, Driguez H, Haser R
J Bacteriol 185 4127-35 (2003)
PMID: 12837787

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153469 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoglucanase G Chains: A, B
Molecule details ›
Chains: A, B
Length: 614 amino acids
Theoretical weight: 68.06 KDa
Source organism: Ruminiclostridium cellulolyticum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37700 (Residues: 36-649; Coverage: 89%)
Gene names: Ccel_0731, celCCG
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand CA 4 x CA
Ligand MG 7 x MG
Ligand EDO 11 x EDO
Ligand GOL 6 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P1
Unit cell:
a: 56.85Å b: 57.72Å c: 86.27Å
α: 94.22° β: 100.87° γ: 99.61°
R-values:
R R work R free
0.174 0.174 0.2
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Clostridium cellulolyticum: model organism of mesophilic cellulolytic clostridia.
Desvaux M. (2005)
3 more

8 mentions without citation

X-Ray crystal structure of the multidomain endoglucanase Cel9G from Clostridium cellulolyticum complexed with natural and synthetic cello-oligosaccharides.
Mandelman et al. (2003)
7 more