› 1g8w

X-ray diffraction
2.8Å resolution

IMPROVED STRUCTURE OF PHYTOHEMAGGLUTININ-L FROM THE KIDNEY BEAN

Released:
DOI: 10.2210/pdb1g8w/pdb
PDB entry 1g8w coloured by chain, front view.
PDB entry 1g8w coloured by chain, side view.
PDB entry 1g8w coloured by chain, top view.
Source organism: Phaseolus vulgaris
Primary publication:
Weak protein-protein interactions in lectins: the crystal structure of a vegetative lectin from the legume Dolichos biflorus.
Buts L, Dao-Thi MH, Loris R, Wyns L, Etzler M, Hamelryck T
J Mol Biol 309 193-201 (2001)
PMID: 11491289

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138408 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Leucoagglutinating phytohemagglutinin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 233 amino acids
Theoretical weight: 25.4 KDa
Source organism: Phaseolus vulgaris
UniProt:
  • Canonical: P05087 (Residues: 21-253; Coverage: 93%)
Gene name: DLEC2
Sequence domains: Legume lectin domain
Structure domains: Jelly Rolls

Ligands and Environments

3 bound ligands:
Ligand NAG 4 x NAG
Ligand MN 4 x MN
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2
Unit cell:
a: 106.479Å b: 121.278Å c: 89.957Å
α: 90° β: 93.18° γ: 90°
R-values:
R R work R free
0.181 0.181 0.212

Quick links

Citations

3 review citations

N-linked oligosaccharides as outfitters for glycoprotein folding, form and function.
Mitra et al. (2006)
2 more

2 mentions without citation

Are Dietary Lectins Relevant Allergens in Plant Food Allergy?
Barre et al. (2020)
1 more