› 1g96

X-ray diffraction
2.5Å resolution

HUMAN CYSTATIN C; DIMERIC FORM WITH 3D DOMAIN SWAPPING

Released:
DOI: 10.2210/pdb1g96/pdb
PDB entry 1g96 coloured by chain, front view.
PDB entry 1g96 coloured by chain, side view.
PDB entry 1g96 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Human cystatin C, an amyloidogenic protein, dimerizes through three-dimensional domain swapping.
Janowski R, Kozak M, Jankowska E, Grzonka Z, Grubb A, Abrahamson M, Jaskolski M
Nat Struct Biol 8 316-20 (2001)
PMID: 11276250

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-133889 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cystatin-C Chain: A
Molecule details ›
Chain: A
Length: 120 amino acids
Theoretical weight: 13.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01034 (Residues: 27-146; Coverage: 100%)
Gene name: CST3
Sequence domains: Cystatin domain
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: I432
Unit cell:
a: 140.53Å b: 140.53Å c: 140.53Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.22 0.249
Expression system: Escherichia coli

Quick links

Citations

43 review citations

Cysteine cathepsins: from structure, function and regulation to new frontiers.
Turk et al. (2012)
42 more

22 mentions without citation

Protein acrobatics in pairs--dimerization via domain swapping.
Gronenborn AM. (2009)
21 more