› 1g9k

X-ray diffraction
1.96Å resolution

CRYSTAL STRUCTURE OF A PSYCHROPHILIC ALKALINE PROTEASE FROM PSEUDOMONAS TAC II 18

Released:
DOI: 10.2210/pdb1g9k/pdb
PDB entry 1g9k coloured by chain, front view.
PDB entry 1g9k coloured by chain, side view.
PDB entry 1g9k coloured by chain, top view.
Source organism: Pseudomonas
Primary publication:
Crystal structures of a psychrophilic metalloprotease reveal new insights into catalysis by cold-adapted proteases.
Aghajari N, Van Petegem F, Villeret V, Chessa JP, Gerday C, Haser R, Van Beeumen J
Proteins 50 636-47 (2003)
PMID: 12577270

Function and Biology Details

Reaction catalysed: 
Preferential cleavage of bonds with hydrophobic residues in P1'.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo trimer
Assembly name:
PDBe Complex ID:
PDB-CPX-130665 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidase metallopeptidase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 463 amino acids
Theoretical weight: 48.73 KDa
Source organism: Pseudomonas
UniProt:
  • Canonical: O69771 (Residues: 18-480; Coverage: 97%)
Gene name: PAPRA
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CA 7 x CA
Ligand SO4 1 x SO4
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FU581
Spacegroup: R3
Unit cell:
a: 186.01Å b: 186.01Å c: 37.97Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.156 0.156 0.187

Quick links

Citations

17 review citations

Psychrophilic enzymes: hot topics in cold adaptation.
Feller et al. (2003)
16 more

6 mentions without citation

Psychrophilic enzymes: from folding to function and biotechnology.
Feller G. (2013)
5 more