› 1g9r

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF GALACTOSYLTRANSFERASE LGTC IN COMPLEX WITH MN AND UDP-2F-GALACTOSE

Released:
DOI: 10.2210/pdb1g9r/pdb
PDB entry 1g9r coloured by chain, front view.
PDB entry 1g9r coloured by chain, side view.
PDB entry 1g9r coloured by chain, top view.
Source organism: Neisseria meningitidis
Primary publication:
Crystal structure of the retaining galactosyltransferase LgtC from Neisseria meningitidis in complex with donor and acceptor sugar analogs.
Persson K, Ly HD, Dieckelmann M, Wakarchuk WW, Withers SG, Strynadka NC
Nat Struct Biol 8 166-75 (2001)
PMID: 11175908

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188049 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
LgtC Chain: A
Molecule details ›
Chain: A
Length: 311 amino acids
Theoretical weight: 36.11 KDa
Source organism: Neisseria meningitidis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q93EK7 (Residues: 1-311; Coverage: 100%)
Gene name: lgtC
Sequence domains: Glycosyl transferase family 8
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

3 bound ligands:
Ligand MN 1 x MN
Ligand UPF 1 x UPF
Ligand ACY 1 x ACY
1 modified residue:
Ligand MSE 7 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL1-5
Spacegroup: P212121
Unit cell:
a: 39.79Å b: 76.05Å c: 86.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.193 0.225
Expression system: Escherichia coli BL21

Quick links

Citations

36 review citations

Lipopolysaccharide endotoxins.
Raetz et al. (2002)
35 more

7 mentions without citation

Three monophyletic superfamilies account for the majority of the known glycosyltransferases.
Liu et al. (2003)
6 more