› 1g9y

X-ray diffraction
2.05Å resolution

HOMING ENDONUCLEASE I-CREI / DNA SUBSTRATE COMPLEX WITH CALCIUM

Released:
DOI: 10.2210/pdb1g9y/pdb
PDB entry 1g9y coloured by chain, front view.
PDB entry 1g9y coloured by chain, side view.
PDB entry 1g9y coloured by chain, top view.
Source organism: Chlamydomonas reinhardtii
Primary publication:
The homing endonuclease I-CreI uses three metals, one of which is shared between the two active sites.
Chevalier BS, Monnat RJ, Stoddard BL
Nat Struct Biol 8 312-6 (2001)
PMID: 11276249

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113858 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA endonuclease I-CreI Chains: A, B
Molecule details ›
Chains: A, B
Length: 152 amino acids
Theoretical weight: 17.52 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P05725 (Residues: 2-153; Coverage: 93%)
Sequence domains: LAGLIDADG endonuclease
Structure domains: Homing endonucleases
5'-D(*CP*GP*AP*AP*AP*CP*TP*GP*TP*CP*TP*CP*AP*CP*GP*AP*CP*GP*TP*TP*TP*TP*GP*C)-3' Chain: C
Molecule details ›
Chain: C
Length: 24 nucleotides
Theoretical weight: 7.32 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Not provided
5'-D(*GP*CP*AP*AP*AP*AP*CP*GP*TP*CP*GP*TP*GP*AP*GP*AP*CP*AP*GP*TP*TP*TP*CP*G)-3' Chain: D
Molecule details ›
Chain: D
Length: 24 nucleotides
Theoretical weight: 7.42 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 43.2Å b: 67.8Å c: 88.3Å
α: 90° β: 91.6° γ: 90°
R-values:
R R work R free
0.207 0.207 0.26
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

13 review citations

Homing endonucleases: structural and functional insight into the catalysts of intron/intein mobility.
Chevalier et al. (2001)
12 more

14 mentions without citation

A novel engineered meganuclease induces homologous recombination in yeast and mammalian cells.
Epinat et al. (2003)
13 more