› 1gc1

X-ray diffraction
2.5Å resolution

HIV-1 GP120 CORE COMPLEXED WITH CD4 AND A NEUTRALIZING HUMAN ANTIBODY

Released:
DOI: 10.2210/pdb1gc1/pdb
Model geometry
Fit model/data
Data not deposited
PDB entry 1gc1 coloured by chain, front view.
PDB entry 1gc1 coloured by chain, side view.
PDB entry 1gc1 coloured by chain, top view.
Source organisms:
Primary publication:
Structure of an HIV gp120 envelope glycoprotein in complex with the CD4 receptor and a neutralizing human antibody.
Kwong PD, Wyatt R, Robinson J, Sweet RW, Sodroski J, Hendrickson WA
Nature 393 648-59 (1998)
PMID: 9641677

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-224968 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (5 distinct):
Surface protein gp120 Chain: G
Molecule details ›
Chain: G
Length: 321 amino acids
Theoretical weight: 35.43 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: P04578 (Residues: 83-127, 195-297, 330-492; Coverage: 38%)
Gene name: env
Sequence domains: Envelope glycoprotein GP120
Structure domains: Human immunodeficiency virus 1, Gp160, envelope glycoprotein
T-cell surface glycoprotein CD4 Chain: C
Molecule details ›
Chain: C
Length: 185 amino acids
Theoretical weight: 20.5 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P01730 (Residues: 26-210; Coverage: 43%)
Gene name: CD4
Sequence domains:
Structure domains: Immunoglobulins
ANTIBODY 17B Chain: L
ANTIBODY 17B Chain: H

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
1 bound ligand:
Ligand NAG 7 x NAG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P2221
Unit cell:
a: 71.64Å b: 88.13Å c: 196.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.21 0.302
Expression systems:
  • Drosophila melanogaster
  • Cricetulus griseus
  • Mus musculus

Quick links

Citations

361 review citations

Receptor binding and membrane fusion in virus entry: the influenza hemagglutinin.
Skehel et al. (2000)
360 more

108 mentions without citation

Probing protein structure by amino acid-specific covalent labeling and mass spectrometry.
Mendoza et al. (2009)
107 more