› 1gc8

X-ray diffraction
2.5Å resolution

THE CRYSTAL STRUCTURE OF THERMUS THERMOPHILUS 3-ISOPROPYLMALATE DEHYDROGENASE MUTATED AT 172TH FROM ALA TO PHE

Released:
DOI: 10.2210/pdb1gc8/pdb
PDB entry 1gc8 coloured by chain, front view.
PDB entry 1gc8 coloured by chain, side view.
PDB entry 1gc8 coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
Design, X-ray crystallography, molecular modelling and thermal stability studies of mutant enzymes at site 172 of 3-isopropylmalate dehydrogenase from Thermus thermophilus.
Qu C, Akanuma S, Tanaka N, Moriyama H, Oshima T
Acta Crystallogr D Biol Crystallogr 57 225-32 (2001)
PMID: 11173468

Function and Biology Details

Reaction catalysed: 
(1a) (2R,3S)-3-isopropylmalate + NAD(+) = (2S)-2-isopropyl-3-oxosuccinate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177982 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-isopropylmalate dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 345 amino acids
Theoretical weight: 36.9 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SIY4 (Residues: 1-345; Coverage: 100%)
Gene names: TTHA1230, leuB
Sequence domains: Isocitrate/isopropylmalate dehydrogenase
Structure domains: Isopropylmalate Dehydrogenase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P21
Unit cell:
a: 55.255Å b: 87.83Å c: 71.186Å
α: 90° β: 100.02° γ: 90°
R-values:
R R work R free
0.17 0.17 0.258
Expression system: Escherichia coli

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Citations

2 review citations

Bacterial Branched-Chain Amino Acid Biosynthesis: Structures, Mechanisms, and Drugability.
Amorim Franco et al. (2017)
1 more