1gdt

X-ray diffraction
3Å resolution

CRYSTAL STRUCTURE OF A SITE-SPECIFIC RECOMBINASE, GAMMA-DELTA RESOLVASE COMPLEXED WITH A 34 BP CLEAVAGE SITE

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-113865 (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
Transposon gamma-delta resolvase Chains: A, B
Molecule details ›
Chains: A, B
Length: 183 amino acids
Theoretical weight: 20.39 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P03012 (Residues: 1-183; Coverage: 100%)
Gene names: ECOK12F009, tnpR
Sequence domains:
Structure domains:
SITE I OF RES DNA Chain: C
Molecule details ›
Chain: C
Length: 22 nucleotides
Theoretical weight: 6.77 KDa
Source organism: Escherichia coli
Expression system: Not provided
SITE I OF RES DNA Chains: D, F
Molecule details ›
Chains: D, F
Length: 13 nucleotides
Theoretical weight: 3.97 KDa
Source organism: Escherichia coli
Expression system: Not provided
SITE I OF RES DNA Chain: E
Molecule details ›
Chain: E
Length: 21 nucleotides
Theoretical weight: 6.45 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 113Å b: 157Å c: 37.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.235 not available
Expression systems:
  • Escherichia coli
  • Not provided