› 1gen

X-ray diffraction
2.15Å resolution

C-TERMINAL DOMAIN OF GELATINASE A

Released:
DOI: 10.2210/pdb1gen/pdb
PDB entry 1gen coloured by chain, front view.
PDB entry 1gen coloured by chain, side view.
PDB entry 1gen coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the haemopexin-like C-terminal domain of gelatinase A.
Libson AM, Gittis AG, Collier IE, Marmer BL, Goldberg GI, Lattman EE
Nat. Struct. Biol. 2 938-42 (1995)
PMID: 7583664

Function and Biology Details

Reaction catalysed: 
Cleavage of gelatin type I and collagen types IV, V, VII, X. Cleaves the collagen-like sequence Pro-Gln-Gly-|-Ile-Ala-Gly-Gln.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140074 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PEX Chain: A
Molecule details ›
Chain: A
Length: 218 amino acids
Theoretical weight: 24.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P08253 (Residues: 443-660; Coverage: 35%)
Gene names: CLG4A, MMP2
Sequence domains: Hemopexin
Structure domains: Hemopexin-like domain

Ligands and Environments

4 bound ligands:
Ligand ZN 1 x ZN
Ligand CA 1 x CA
Ligand CL 1 x CL
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 57.9Å b: 77.4Å c: 53.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.188 0.26
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Progress in matrix metalloproteinase research.
Murphy et al. (2008)
11 more

11 mentions without citation

A unified statistical model to support local sequence order independent similarity searching for ligand-binding sites and its application to genome-based drug discovery.
Xie et al. (2009)
10 more