› 1ggx

X-ray diffraction
1.9Å resolution

RED FLUORESCENT PROTEIN (FP583 OR DSRED(CLONTECH)) FROM DISCOSOMA SP.

Released:
DOI: 10.2210/pdb1ggx/pdb
PDB entry 1ggx coloured by chain, front view.
PDB entry 1ggx coloured by chain, side view.
PDB entry 1ggx coloured by chain, top view.
Source organism: Discosoma sp.
Primary publication:
The structural basis for red fluorescence in the tetrameric GFP homolog DsRed.
Wall MA, Socolich M, Ranganathan R
Nat Struct Biol 7 1133-8 (2000)
PMID: 11101896

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193624 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Red fluorescent protein drFP583 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 223 amino acids
Theoretical weight: 25.95 KDa
Source organism: Discosoma sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9U6Y8 (Residues: 1-225; Coverage: 100%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CRQ 4 x CRQ

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 56.206Å b: 129.621Å c: 57.441Å
α: 90° β: 99.15° γ: 90°
R-values:
R R work R free
0.192 0.192 0.212
Expression system: Escherichia coli

Quick links

Citations

25 review citations

Fluorescent proteins and their applications in imaging living cells and tissues.
Chudakov et al. (2010)
24 more

10 mentions without citation

Nanobodies: Chemical Functionalization Strategies and Intracellular Applications.
Schumacher et al. (2018)
9 more