› 1gir

X-ray diffraction
2.1Å resolution

CRYSTAL STRUCTURE OF THE ENZYMATIC COMPONET OF IOTA-TOXIN FROM CLOSTRIDIUM PERFRINGENS WITH NADPH

Released:
DOI: 10.2210/pdb1gir/pdb
PDB entry 1gir coloured by chain, front view.
PDB entry 1gir coloured by chain, side view.
PDB entry 1gir coloured by chain, top view.
Source organism: Clostridium perfringens
Primary publication:
Crystal structure and site-directed mutagenesis of enzymatic components from Clostridium perfringens iota-toxin.
Tsuge H, Nagahama M, Nishimura H, Hisatsune J, Sakaguchi Y, Itogawa Y, Katunuma N, Sakurai J
J Mol Biol 325 471-83 (2003)
PMID: 12498797

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175104 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ADP ribosyltransferase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 413 amino acids
Theoretical weight: 47.65 KDa
Source organism: Clostridium perfringens
UniProt:
  • Canonical: Q46220 (Residues: 42-454; Coverage: 91%)
Sequence domains: ADP-ribosyltransferase exoenzyme
Structure domains: Toxin ADP-ribosyltransferase; Chain A, domain 1

Ligands and Environments

1 bound ligand:
Ligand NDP 1 x NDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-18B
Spacegroup: P212121
Unit cell:
a: 59.6Å b: 88.37Å c: 93.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.205 0.248

Quick links

Citations

21 review citations

Binary bacterial toxins: biochemistry, biology, and applications of common Clostridium and Bacillus proteins.
Barth et al. (2004)
20 more

1 mention without citation

Host cell cytotoxicity and cytoskeleton disruption by CerADPr, an ADP-ribosyltransferase of Bacillus cereus G9241.
Simon et al. (2013)