› 1gk0

X-ray diffraction
2.5Å resolution

Structure-based prediction of modifications in glutarylamidase to allow single-step enzymatic production of 7-aminocephalosporanic acid from cephalosporin C

Released:
DOI: 10.2210/pdb1gk0/pdb
PDB entry 1gk0 coloured by chain, front view.
PDB entry 1gk0 coloured by chain, side view.
PDB entry 1gk0 coloured by chain, top view.
Source organism: Pseudomonas sp.
Primary publication:
Structure-based prediction of modifications in glutarylamidase to allow single-step enzymatic production of 7-aminocephalosporanic acid from cephalosporin C.
Fritz-Wolf K, Koller KP, Lange G, Liesum A, Sauber K, Schreuder H, Aretz W, Kabsch W
Protein Sci 11 92-103 (2002)
PMID: 11742126

Function and Biology Details

Reaction catalysed: 
(7R)-7-(4-carboxybutanamido)cephalosporanate + H(2)O = (7R)-7-aminocephalosporanate + glutarate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139685 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Glutaryl-7-aminocephalosporanic-acid acylase subunit alpha Chains: A, C
Molecule details ›
Chains: A, C
Length: 153 amino acids
Theoretical weight: 16.99 KDa
Source organism: Pseudomonas sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: P07662 (Residues: 36-188; Coverage: 22%)
Structure domains: Penicillin Amidohydrolase, domain 1
Glutaryl-7-aminocephalosporanic-acid acylase subunit beta Chains: B, D
Molecule details ›
Chains: B, D
Length: 522 amino acids
Theoretical weight: 58.86 KDa
Source organism: Pseudomonas sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: P07662 (Residues: 199-720; Coverage: 76%)
Sequence domains: Penicillin amidase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand PO4 2 x PO4
Ligand EDO 2 x EDO
1 modified residue:
Ligand MSE 24 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: C2
Unit cell:
a: 228.366Å b: 69.91Å c: 113.55Å
α: 90° β: 97.57° γ: 90°
R-values:
R R work R free
0.207 0.207 0.222
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Cephalosporin C acylase: dream and(/or) reality.
Pollegioni et al. (2013)
2 more

3 mentions without citation

Structure-based prediction of modifications in glutarylamidase to allow single-step enzymatic production of 7-aminocephalosporanic acid from cephalosporin C.
Fritz-Wolf et al. (2002)
2 more