› 1gkk

X-ray diffraction
1.6Å resolution

Feruloyl esterase domain of XynY from clostridium thermocellum

Released:
DOI: 10.2210/pdb1gkk/pdb
PDB entry 1gkk coloured by chain, front view.
PDB entry 1gkk coloured by chain, side view.
PDB entry 1gkk coloured by chain, top view.
Source organism: Acetivibrio thermocellus
Primary publication:
The structure of the feruloyl esterase module of xylanase 10B from Clostridium thermocellum provides insights into substrate recognition.
Prates JA, Tarbouriech N, Charnock SJ, Fontes CM, Ferreira LM, Davies GJ
Structure 9 1183-90 (2001)
PMID: 11738044

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156366 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endo-1,4-beta-xylanase Y Chains: A, B
Molecule details ›
Chains: A, B
Length: 297 amino acids
Theoretical weight: 34.27 KDa
Source organism: Acetivibrio thermocellus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P51584 (Residues: 792-1077; Coverage: 27%)
Gene name: xynY
Sequence domains: Putative esterase
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
Ligand CD 10 x CD
Ligand GOL 1 x GOL
2 modified residues:
Ligand MSE 18 x MSE
Ligand SEP 2 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 65.381Å b: 108.793Å c: 113.877Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.155 0.154 0.173
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

Microbial hemicellulases.
Shallom et al. (2003)
12 more

3 mentions without citation

ID30A-3 (MASSIF-3) - a beamline for macromolecular crystallography at the ESRF with a small intense beam.
von Stetten et al. (2020)
2 more