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X-ray diffraction
1Å resolution

HISTIDINE AMMONIA-LYASE (HAL) FROM PSEUDOMONAS PUTIDA INHIBITED WITH L-CYSTEINE

Released:
DOI: 10.2210/pdb1gkm/pdb
PDB entry 1gkm coloured by chain, front view.
PDB entry 1gkm coloured by chain, side view.
PDB entry 1gkm coloured by chain, top view.
Source organism: Pseudomonas putida
Primary publication:
Structures of two histidine ammonia-lyase modifications and implications for the catalytic mechanism.
Baedeker M, Schulz GE
Eur J Biochem 269 1790-7 (2002)
PMID: 11895450

Function and Biology Details

Reaction catalysed: 
L-histidine = urocanate + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149201 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Histidine ammonia-lyase Chain: A
Molecule details ›
Chain: A
Length: 507 amino acids
Theoretical weight: 53.62 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P21310 (Residues: 2-510; Coverage: 100%)
Gene name: hutH
Sequence domains: Aromatic amino acid lyase
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand CYS 1 x CYS
Ligand O 1 x O
Ligand SO4 1 x SO4
Ligand GOL 1 x GOL
1 modified residue:
Ligand MDO 1 x MDO

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: I222
Unit cell:
a: 79.267Å b: 116.788Å c: 129.532Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.119 not available 0.135
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

New generation of biocatalysts for organic synthesis.
Nestl et al. (2014)
2 more

8 mentions without citation

Discovery of two cyanobacterial phenylalanine ammonia lyases: kinetic and structural characterization.
Moffitt et al. (2007)
7 more