› 1gn2

X-ray diffraction
3.4Å resolution

S123C mutant of the iron-superoxide dismutase from Mycobacterium tuberculosis.

Released:
DOI: 10.2210/pdb1gn2/pdb
PDB entry 1gn2 coloured by chain, front view.
PDB entry 1gn2 coloured by chain, side view.
PDB entry 1gn2 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Engineering of an intersubunit disulfide bridge in the iron-superoxide dismutase of Mycobacterium tuberculosis.
Bunting KA, Cooper JB, Tickle IJ, Young DB
Arch Biochem Biophys 397 69-76 (2002)
PMID: 11747311

Function and Biology Details

Reaction catalysed: 
2 superoxide + 2 H(+) = O(2) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161470 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Superoxide dismutase [Fe] Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 207 amino acids
Theoretical weight: 23.08 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Mycolicibacterium vaccae
UniProt:
  • Canonical: P9WGE7 (Residues: 1-207; Coverage: 100%)
Gene names: MTCY01A6.22c, Rv3846, sod, sodA, sodB
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand FE 8 x FE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR591
Spacegroup: P21
Unit cell:
a: 103.08Å b: 106.33Å c: 76.1Å
α: 90° β: 92.37° γ: 90°
R-values:
R R work R free
0.249 0.249 0.27
Expression system: Mycolicibacterium vaccae

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Citations

1 review citation

FoldX as Protein Engineering Tool: Better Than Random Based Approaches?
Buß et al. (2018)
12 other articles