› 1gof

X-ray diffraction
1.7Å resolution

NOVEL THIOETHER BOND REVEALED BY A 1.7 ANGSTROMS CRYSTAL STRUCTURE OF GALACTOSE OXIDASE

Released:
DOI: 10.2210/pdb1gof/pdb
PDB entry 1gof coloured by chain, front view.
PDB entry 1gof coloured by chain, side view.
PDB entry 1gof coloured by chain, top view.
Source organism: Hypomyces rosellus
Primary publication:
Novel thioether bond revealed by a 1.7 A crystal structure of galactose oxidase.
Ito N, Phillips SE, Stevens C, Ogel ZB, McPherson MJ, Keen JN, Yadav KD, Knowles PF
Nature 350 87-90 (1991)
PMID: 2002850

Function and Biology Details

Reaction catalysed: 
D-galactose + O(2) = D-galacto-hexodialdose + H(2)O(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143550 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Galactose oxidase Chain: A
Molecule details ›
Chain: A
Length: 639 amino acids
Theoretical weight: 68.58 KDa
Source organism: Hypomyces rosellus
Expression system: Not provided
UniProt:
  • Canonical: P0CS93 (Residues: 42-680; Coverage: 97%)
Gene name: GAOA
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CU 1 x CU
Ligand NA 1 x NA
Ligand ACY 2 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2
Unit cell:
a: 98Å b: 89.4Å c: 86.7Å
α: 90° β: 117.8° γ: 90°
R-values:
R R work R free
0.177 not available not available
Expression system: Not provided

Quick links

Citations

60 review citations

Aerobic copper-catalyzed organic reactions.
Allen et al. (2013)
59 more

30 mentions without citation

Copper active sites in biology.
Solomon et al. (2014)
29 more