› 1gp2

X-ray diffraction
2.3Å resolution

G PROTEIN HETEROTRIMER GI_ALPHA_1 BETA_1 GAMMA_2 WITH GDP BOUND

Released:
DOI: 10.2210/pdb1gp2/pdb
PDB entry 1gp2 coloured by chain, front view.
PDB entry 1gp2 coloured by chain, side view.
PDB entry 1gp2 coloured by chain, top view.
Source organisms:
Primary publication:
The structure of the G protein heterotrimer Gi alpha 1 beta 1 gamma 2.
Wall MA, Coleman DE, Lee E, Iñiguez-Lluhi JA, Posner BA, Gilman AG, Sprang SR
Cell 83 1047-58 (1995)
PMID: 8521505

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145473 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 353 amino acids
Theoretical weight: 40.27 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P10824 (Residues: 2-354; Coverage: 100%)
Gene names: Gnai-1, Gnai1
Sequence domains: G-protein alpha subunit
Structure domains:
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 340 amino acids
Theoretical weight: 37.42 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: P62871 (Residues: 1-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.85 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63212 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Structure domains: Transducin (heterotrimeric G protein), gamma chain

Ligands and Environments

1 bound ligand:
Ligand GDP 1 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P43
Unit cell:
a: 84.289Å b: 84.289Å c: 132.387Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.226 0.307
Expression systems:
  • Escherichia coli
  • Not provided
  • Spodoptera frugiperda

Quick links

Citations

131 review citations

Integrins: bidirectional, allosteric signaling machines.
Hynes RO. (2002)
130 more

129 mentions without citation

Heterotrimeric G-proteins: a short history.
Milligan et al. (2006)
128 more