› 1gp5

X-ray diffraction
2.2Å resolution

Anthocyanidin synthase from Arabidopsis thaliana complexed with trans-dihydroquercetin

Released:
DOI: 10.2210/pdb1gp5/pdb
PDB entry 1gp5 coloured by chain, front view.
PDB entry 1gp5 coloured by chain, side view.
PDB entry 1gp5 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structure and mechanism of anthocyanidin synthase from Arabidopsis thaliana.
Wilmouth RC, Turnbull JJ, Welford RW, Clifton IJ, Prescott AG, Schofield CJ
Structure 10 93-103 (2002)
PMID: 11796114

Function and Biology Details

Reaction catalysed: 
(1a) a (2R,3S,4S)-leucoanthocyanidin + 2-oxoglutarate + O(2) = a (4S)- 2,3-dehydroflavan-3,4-diol + succinate + CO(2) + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188303 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Leucoanthocyanidin dioxygenase Chain: A
Molecule details ›
Chain: A
Length: 356 amino acids
Theoretical weight: 40.45 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96323 (Residues: 1-356; Coverage: 100%)
Gene names: At4g22880, F7H19.60, LDOX
Sequence domains:
Structure domains: B-lactam Antibiotic, Isopenicillin N Synthase; Chain

Ligands and Environments

5 bound ligands:
Ligand AKG 1 x AKG
Ligand MES 2 x MES
Ligand DQH 1 x DQH
Ligand DH2 1 x DH2
Ligand FE 1 x FE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P212121
Unit cell:
a: 62.033Å b: 74.362Å c: 88.109Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.204 0.237
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

23 review citations

Oxygen sensing by HIF hydroxylases.
Schofield et al. (2004)
22 more

11 mentions without citation

Structure and function of enzymes involved in the biosynthesis of phenylpropanoids.
Ferrer et al. (2008)
10 more