› 1gqi

X-ray diffraction
1.48Å resolution

Structure of Pseudomonas cellulosa alpha-D-glucuronidase

Released:
DOI: 10.2210/pdb1gqi/pdb
PDB entry 1gqi coloured by chain, front view.
PDB entry 1gqi coloured by chain, side view.
PDB entry 1gqi coloured by chain, top view.
Source organism: Cellvibrio japonicus
Primary publication:
The structural basis for catalysis and specificity of the Pseudomonas cellulosa alpha-glucuronidase, GlcA67A.
Nurizzo D, Nagy T, Gilbert HJ, Davies GJ
Structure 10 547-56 (2002)
PMID: 11937059

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->2)-alpha-D-(4-O-methyl)glucuronosyl links in the main chain of hardwood xylans
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109885 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Extracellular xylan exo-alpha-(1->2)-glucuronosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 708 amino acids
Theoretical weight: 80.44 KDa
Source organism: Cellvibrio japonicus
Expression system: Escherichia coli
UniProt:
  • Canonical: B3PC73 (Residues: 25-732; Coverage: 99%)
Gene names: CJA_2887, gla67A, glcA67A
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand EDO 12 x EDO
Ligand CO 8 x CO
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P1
Unit cell:
a: 69.912Å b: 74.682Å c: 87.587Å
α: 115.18° β: 93.14° γ: 109.33°
R-values:
R R work R free
0.133 0.133 0.168
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Microbial hemicellulases.
Shallom et al. (2003)
6 more

4 mentions without citation

Effect of dimer dissociation on activity and thermostability of the alpha-glucuronidase from Geobacillus stearothermophilus: dissecting the different oligomeric forms of family 67 glycoside hydrolases.
Shallom et al. (2004)
3 more