› 1gqk

X-ray diffraction
1.9Å resolution

Structure of Pseudomonas cellulosa alpha-D-glucuronidase complexed with glucuronic acid

Released:
DOI: 10.2210/pdb1gqk/pdb
PDB entry 1gqk coloured by chain, front view.
PDB entry 1gqk coloured by chain, side view.
PDB entry 1gqk coloured by chain, top view.
Source organism: Cellvibrio japonicus
Primary publication:
The structural basis for catalysis and specificity of the Pseudomonas cellulosa alpha-glucuronidase, GlcA67A.
Nurizzo D, Nagy T, Gilbert HJ, Davies GJ
Structure 10 547-56 (2002)
PMID: 11937059

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->2)-alpha-D-(4-O-methyl)glucuronosyl links in the main chain of hardwood xylans
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109885 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Extracellular xylan exo-alpha-(1->2)-glucuronosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 708 amino acids
Theoretical weight: 80.44 KDa
Source organism: Cellvibrio japonicus
Expression system: Escherichia coli
UniProt:
  • Canonical: B3PC73 (Residues: 25-732; Coverage: 99%)
Gene names: CJA_2887, gla67A, glcA67A
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand BDP 2 x BDP
Ligand EDO 12 x EDO
Ligand CO 8 x CO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P1
Unit cell:
a: 69.707Å b: 74.879Å c: 87.481Å
α: 115.34° β: 93.06° γ: 109.2°
R-values:
R R work R free
0.152 0.151 0.186
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Microbial hemicellulases.
Shallom et al. (2003)
6 more