› 1gqq

X-ray diffraction
3.1Å resolution

MURC - Crystal structure of the apo-enzyme from Haemophilus influenzae

Released:
DOI: 10.2210/pdb1gqq/pdb
PDB entry 1gqq coloured by chain, front view.
PDB entry 1gqq coloured by chain, side view.
PDB entry 1gqq coloured by chain, top view.
Source organism: Haemophilus influenzae
Entry authors: Skarzynski T, Cleasby A, Domenici E, Gevi M, Shaw J

Function and Biology Details

Reaction catalysed: 
ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155244 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylmuramate--L-alanine ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 475 amino acids
Theoretical weight: 52.06 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P45066 (Residues: 1-475; Coverage: 100%)
Gene names: HI_1139, murC
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-BM
Spacegroup: P212121
Unit cell:
a: 65.508Å b: 99.485Å c: 180.59Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.239 0.286
Expression system: Escherichia coli BL21

Quick links