› 1gsk

X-ray diffraction
1.7Å resolution

Crystal structure of CotA, an endospore coat protein from Bacillus subtilis

Released:
DOI: 10.2210/pdb1gsk/pdb
PDB entry 1gsk coloured by chain, front view.
PDB entry 1gsk coloured by chain, side view.
PDB entry 1gsk coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Crystal structure of a bacterial endospore coat component. A laccase with enhanced thermostability properties.
Enguita FJ, Martins LO, Henriques AO, Carrondo MA
J Biol Chem 278 19416-25 (2003)
PMID: 12637519

Function and Biology Details

Reactions catalysed: 
4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O
2 bilirubin + O(2) = 2 biliverdin + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139758 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Laccase Chain: A
Molecule details ›
Chain: A
Length: 513 amino acids
Theoretical weight: 58.57 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P07788 (Residues: 1-513; Coverage: 100%)
Gene names: BSU06300, cotA, pig
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

4 bound ligands:
Ligand GOL 4 x GOL
Ligand CU 1 x CU
Ligand C2O 1 x C2O
Ligand C1O 1 x C1O
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P3121
Unit cell:
a: 102.051Å b: 102.051Å c: 136.393Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.179 0.178 0.199
Expression system: Escherichia coli BL21

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Citations

27 review citations

Fungal laccases - occurrence and properties.
Baldrian P. (2006)
26 more

21 mentions without citation

Designing Artificial Metalloenzymes by Tuning of the Environment beyond the Primary Coordination Sphere.
Van Stappen et al. (2022)
20 more