› 1gsq

X-ray diffraction
2.4Å resolution

THREE-DIMENSIONAL STRUCTURE, CATALYTIC PROPERTIES AND EVOLUTION OF A SIGMA CLASS GLUTATHIONE TRANSFERASE FROM SQUID, A PROGENITOR OF THE LENS-CRYSTALLINS OF CEPHALOPODS

Released:
DOI: 10.2210/pdb1gsq/pdb
PDB entry 1gsq coloured by chain, front view.
PDB entry 1gsq coloured by chain, side view.
PDB entry 1gsq coloured by chain, top view.
Source organism: Todarodes pacificus
Primary publication:
Three-dimensional structure, catalytic properties, and evolution of a sigma class glutathione transferase from squid, a progenitor of the lens S-crystallins of cephalopods.
Ji X, von Rosenvinge EC, Johnson WW, Tomarev SI, Piatigorsky J, Armstrong RN, Gilliland GL
Biochemistry 34 5317-28 (1995)
PMID: 7727393

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155424 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase Chain: A
Molecule details ›
Chain: A
Length: 202 amino acids
Theoretical weight: 22.82 KDa
Source organism: Todarodes pacificus
Expression system: Escherichia coli
UniProt:
  • Canonical: P46088 (Residues: 2-203; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand GDN 1 x GDN
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P3121
Unit cell:
a: 72.76Å b: 72.76Å c: 94.63Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.18 not available not available
Expression system: Escherichia coli

Quick links

Citations

13 review citations

The glutathione S-transferase supergene family: regulation of GST and the contribution of the isoenzymes to cancer chemoprotection and drug resistance.
Hayes et al. (1995)
12 more

8 mentions without citation

The molecular refractive function of lens γ-Crystallins.
Zhao et al. (2011)
7 more