› 1gt0

X-ray diffraction
2.6Å resolution

Crystal structure of a POU/HMG/DNA ternary complex

Released:
DOI: 10.2210/pdb1gt0/pdb
PDB entry 1gt0 coloured by chain, front view.
PDB entry 1gt0 coloured by chain, side view.
PDB entry 1gt0 coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of a POU/HMG/DNA ternary complex suggests differential assembly of Oct4 and Sox2 on two enhancers.
Reményi A, Lins K, Nissen LJ, Reinbold R, Schöler HR, Wilmanns M
Genes Dev 17 2048-59 (2003)
PMID: 12923055

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113876 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
POU domain, class 2, transcription factor 1 Chain: C
Molecule details ›
Chain: C
Length: 159 amino acids
Theoretical weight: 18.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P14859 (Residues: 280-438; Coverage: 21%)
Gene names: OCT1, OTF1, POU2F1
Sequence domains:
Structure domains:
Transcription factor SOX-2 Chain: D
Molecule details ›
Chain: D
Length: 80 amino acids
Theoretical weight: 9.83 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P48432 (Residues: 41-120; Coverage: 25%)
Gene names: Sox-2, Sox2
Sequence domains: HMG (high mobility group) box
Structure domains: High mobility group box domain
5'-D(*TP*TP*CP*TP*TP*TP*GP*TP*TP*TP* GP*GP*AP* TP*GP*CP*TP*AP*AP*TP*GP*GP*GP*A)-3' Chain: A
Molecule details ›
Chain: A
Length: 24 nucleotides
Theoretical weight: 7.44 KDa
5'-D(*AP*TP*CP*CP*CP*AP*TP*TP*AP*GP* CP*AP*TP*CP*CP*AP*AP*AP*CP*AP*AP*AP*GP*A)-3' Chain: B
Molecule details ›
Chain: B
Length: 24 nucleotides
Theoretical weight: 7.3 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P3121
Unit cell:
a: 72.8Å b: 72.8Å c: 172.4Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.232 0.232 0.285
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

42 review citations

Origins of specificity in protein-DNA recognition.
Rohs et al. (2010)
41 more

40 mentions without citation

Homeodomain revisited: a lesson from disease-causing mutations.
Chi YI. (2005)
39 more