› 1guk

X-ray diffraction
2.9Å resolution

CRYSTAL STRUCTURE OF MURINE ALPHA-CLASS GSTA4-4

Released:
DOI: 10.2210/pdb1guk/pdb
PDB entry 1guk coloured by chain, front view.
PDB entry 1guk coloured by chain, side view.
PDB entry 1guk coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structure of a murine alpha-class glutathione S-transferase involved in cellular defense against oxidative stress.
Krengel U, Schröter KH, Hoier H, Arkema A, Kalk KH, Zimniak P, Dijkstra BW
FEBS Lett 422 285-90 (1998)
PMID: 9498801

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150234 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase A4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 222 amino acids
Theoretical weight: 25.61 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P24472 (Residues: 1-222; Coverage: 100%)
Gene names: Gsta, Gsta4
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.5
Spacegroup: P21212
Unit cell:
a: 114.3Å b: 95.9Å c: 50.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.234 not available
Expression system: Escherichia coli

Quick links

Citations

9 citation in other articles

Human glutathione transferase A4-4 crystal structures and mutagenesis reveal the basis of high catalytic efficiency with toxic lipid peroxidation products.
Bruns et al. (1999)
8 more

2 mentions without citation

Binding of hematin by a new class of glutathione transferase from the blood-feeding parasitic nematode Haemonchus contortus.
van Rossum et al. (2004)
1 more