› 1guz

X-ray diffraction
2Å resolution

Structural Basis for Thermophilic Protein Stability: Structures of Thermophilic and Mesophilic Malate Dehydrogenases

Released:
DOI: 10.2210/pdb1guz/pdb
PDB entry 1guz coloured by chain, front view.
PDB entry 1guz coloured by chain, side view.
PDB entry 1guz coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for thermophilic protein stability: structures of thermophilic and mesophilic malate dehydrogenases.
Dalhus B, Saarinen M, Sauer UH, Eklund P, Johansson K, Karlsson A, Ramaswamy S, Bjørk A, Synstad B, Naterstad K, Sirevåg R, Eklund H
J Mol Biol 318 707-21 (2002)
PMID: 12054817

Function and Biology Details

Reaction catalysed: 
(S)-malate + NAD(+) = oxaloacetate + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143169 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Malate dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 310 amino acids
Theoretical weight: 33.31 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P80039 (Residues: 1-73, 227-229; Coverage: 25%)
  • Canonical: P0C890 (Residues: 74-226, 230-310; Coverage: 76%)
Gene names: CT1507, mdh
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P212121
Unit cell:
a: 83.89Å b: 117.39Å c: 125.33Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.243 0.243 0.292
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Rational engineering of enzyme stability.
Eijsink et al. (2004)
3 more

2 mentions without citation

"Hot cores" in proteins: comparative analysis of the apolar contact area in structures from hyper/thermophilic and mesophilic organisms.
Paiardini et al. (2008)
1 more