› 1gvk

X-ray diffraction
0.94Å resolution

Porcine pancreatic elastase acyl enzyme at 0.95 A resolution

Released:
DOI: 10.2210/pdb1gvk/pdb
PDB entry 1gvk coloured by chain, front view.
PDB entry 1gvk coloured by chain, side view.
PDB entry 1gvk coloured by chain, top view.
Source organisms:
Primary publication:
X-ray structure of a serine protease acyl-enzyme complex at 0.95-A resolution.
Katona G, Wilmouth RC, Wright PA, Berglund GI, Hajdu J, Neutze R, Schofield CJ
J Biol Chem 277 21962-70 (2002)
PMID: 11896054

Function and Biology Details

Reaction catalysed: 
Hydrolysis of proteins, including elastin. Preferential cleavage: Ala-|-.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-133456 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
PEPTIDE INHIBITOR Chain: A
Molecule details ›
Chain: A
Length: 4 amino acids
Theoretical weight: 368 Da
Source organism: synthetic construct
Expression system: Not provided
Chymotrypsin-like elastase family member 1 Chain: B
Molecule details ›
Chain: B
Length: 240 amino acids
Theoretical weight: 25.93 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P00772 (Residues: 27-266; Coverage: 96%)
Gene names: CELA1, ELA1
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P212121
Unit cell:
a: 50.176Å b: 57.778Å c: 74.409Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.122 not available 0.147
Expression system: Not provided

Quick links

Citations

4 review citations

Unconventional serine proteases: variations on the catalytic Ser/His/Asp triad configuration.
Ekici et al. (2008)
3 more

5 mentions without citation

Insights into the serine protease mechanism from atomic resolution structures of trypsin reaction intermediates.
Radisky et al. (2006)
4 more