› 1gwj

X-ray diffraction
2.2Å resolution

Morphinone reductase

Released:
DOI: 10.2210/pdb1gwj/pdb
PDB entry 1gwj coloured by chain, front view.
PDB entry 1gwj coloured by chain, side view.
PDB entry 1gwj coloured by chain, top view.
Source organism: Pseudomonas putida
Primary publication:
Crystal structure of bacterial morphinone reductase and properties of the C191A mutant enzyme.
Barna T, Messiha HL, Petosa C, Bruce NC, Scrutton NS, Moody PC
J Biol Chem 277 30976-83 (2002)
PMID: 12048188

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175995 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 377 amino acids
Theoretical weight: 41.22 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
  • Canonical: Q51990 (Residues: 1-377; Coverage: 100%)
Gene name: morB
Sequence domains: NADH:flavin oxidoreductase / NADH oxidase family
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
Ligand FMN 1 x FMN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: I212121
Unit cell:
a: 49.523Å b: 121.341Å c: 178.215Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.206 0.216
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Asymmetric bioreduction of activated C=C bonds using enoate reductases from the old yellow enzyme family.
Stuermer et al. (2007)
2 more

8 mentions without citation

Hydrogen tunnelling in enzyme-catalysed H-transfer reactions: flavoprotein and quinoprotein systems.
Sutcliffe et al. (2006)
7 more