› 1gww

X-ray diffraction
1.8Å resolution

ALPHA-,1,3 GALACTOSYLTRANSFERASE - ALPHA-D-GLUCOSE COMPLEX

Released:
DOI: 10.2210/pdb1gww/pdb
PDB entry 1gww coloured by chain, front view.
PDB entry 1gww coloured by chain, side view.
PDB entry 1gww coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Structural basis of ordered binding of donor and acceptor substrates to the retaining glycosyltransferase, alpha-1,3-galactosyltransferase.
Boix E, Zhang Y, Swaminathan GJ, Brew K, Acharya KR
J Biol Chem 277 28310-8 (2002)
PMID: 12011052

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-galactose + beta-D-galactosyl-(1->4)-beta-N-acetyl-D-glucosaminyl-R = UDP + alpha-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-N-acetylglucosaminyl-R
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147073 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acetyllactosaminide alpha-1,3-galactosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 289 amino acids
Theoretical weight: 34.09 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P14769 (Residues: 80-368; Coverage: 79%)
Gene name: GGTA1
Sequence domains: Glycosyltransferase family 6
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

3 bound ligands:
Ligand UDP 2 x UDP
Ligand MN 2 x MN
Ligand GLC 1 x GLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P21
Unit cell:
a: 45.163Å b: 94.37Å c: 94.76Å
α: 90° β: 99.4° γ: 90°
R-values:
R R work R free
0.21 0.21 0.232
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Structures and mechanisms of glycosyltransferases.
Breton et al. (2006)
11 more

2 mentions without citation

Automated identification of protein-ligand interaction features using Inductive Logic Programming: a hexose binding case study.
A Santos et al. (2012)
1 more