› 1gwy

X-ray diffraction
1.71Å resolution

Crystal structure of the water-soluble state of the pore-forming cytolysin Sticholysin II

Released:
DOI: 10.2210/pdb1gwy/pdb
PDB entry 1gwy coloured by chain, front view.
PDB entry 1gwy coloured by chain, side view.
PDB entry 1gwy coloured by chain, top view.
Source organism: Stichodactyla helianthus
Primary publication:
Crystal and electron microscopy structures of sticholysin II actinoporin reveal insights into the mechanism of membrane pore formation.
Mancheño JM, Martín-Benito J, Martínez-Ripoll M, Gavilanes JG, Hermoso JA
Structure 11 1319-28 (2003)
PMID: 14604522

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139789 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DELTA-stichotoxin-She4b Chains: A, B
Molecule details ›
Chains: A, B
Length: 175 amino acids
Theoretical weight: 19.3 KDa
Source organism: Stichodactyla helianthus
UniProt:
  • Canonical: P07845 (Residues: 1-175; Coverage: 100%)
Sequence domains: Sea anemone cytotoxic protein
Structure domains: Cytolysin/lectin

Ligands and Environments

1 bound ligand:
Ligand SO4 8 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P21
Unit cell:
a: 32.304Å b: 119.735Å c: 43.421Å
α: 90° β: 90.04° γ: 90°
R-values:
R R work R free
0.216 0.216 0.256

Quick links

Citations

28 review citations

Pore-forming toxins: ancient, but never really out of fashion.
Dal Peraro et al. (2016)
27 more

21 mentions without citation

Sea anemone (Cnidaria, Anthozoa, Actiniaria) toxins: an overview.
Frazão et al. (2012)
20 more