1gxm

X-ray diffraction
1.32Å resolution

Family 10 polysaccharide lyase from Cellvibrio cellulosa

Released:

Function and Biology Details

Reaction catalysed:
Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190267 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pectate lyase Chains: A, B
Molecule details ›
Chains: A, B
Length: 332 amino acids
Theoretical weight: 36.71 KDa
Source organism: Cellvibrio japonicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9F7L3 (Residues: 323-649; Coverage: 53%)
Gene name: Pel10A
Sequence domains: Pectic acid lyase
Structure domains: Glycosyltransferase

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 47.67Å b: 106.113Å c: 55.365Å
α: 90° β: 91.95° γ: 90°
R-values:
R R work R free
0.132 0.13 0.162
Expression system: Escherichia coli