› 1gy5

X-ray diffraction
2.3Å resolution

D92N,D94N double point mutant of human Nuclear Transport Factor 2 (NTF2)

Released:
DOI: 10.2210/pdb1gy5/pdb
PDB entry 1gy5 coloured by chain, front view.
PDB entry 1gy5 coloured by chain, side view.
PDB entry 1gy5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for the interaction between NTF2 and nucleoporin FxFG repeats.
Bayliss R, Leung SW, Baker RP, Quimby BB, Corbett AH, Stewart M
EMBO J 21 2843-53 (2002)
PMID: 12065398

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158511 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nuclear transport factor 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 127 amino acids
Theoretical weight: 14.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61970 (Residues: 1-127; Coverage: 100%)
Gene names: NTF2, NUTF2
Sequence domains: Nuclear transport factor 2 (NTF2) domain
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 35.04Å b: 79.02Å c: 42.14Å
α: 90° β: 104.36° γ: 90°
R-values:
R R work R free
0.222 0.222 0.264
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

31 review citations

Molecular mechanism of the nuclear protein import cycle.
Stewart M. (2007)
30 more

5 mentions without citation

An overview on the recently discovered iota-carbonic anhydrases.
Nocentini et al. (2021)
4 more