› 1gy8

X-ray diffraction
2Å resolution

Trypanosoma brucei UDP-galactose 4' epimerase

Released:
DOI: 10.2210/pdb1gy8/pdb
PDB entry 1gy8 coloured by chain, front view.
PDB entry 1gy8 coloured by chain, side view.
PDB entry 1gy8 coloured by chain, top view.
Source organism: Trypanosoma brucei
Primary publication:
High-resolution crystal structure of Trypanosoma brucei UDP-galactose 4'-epimerase: a potential target for structure-based development of novel trypanocides.
Shaw MP, Bond CS, Roper JR, Gourley DG, Ferguson MA, Hunter WN
Mol Biochem Parasitol 126 173-80 (2003)
PMID: 12615316

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-glucose = UDP-alpha-D-galactose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186145 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-glucose 4-epimerase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 397 amino acids
Theoretical weight: 43.81 KDa
Source organism: Trypanosoma brucei
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8T8E9 (Residues: 1-395; Coverage: 100%)
Gene name: galE
Sequence domains: NAD dependent epimerase/dehydratase family
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
1 bound ligand:
Ligand UDP 4 x UDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 102.185Å b: 112.533Å c: 160.591Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.216 0.245
Expression system: Escherichia coli BL21

Quick links

Citations

25 citation in other articles

Sugar nucleotide pools of Trypanosoma brucei, Trypanosoma cruzi, and Leishmania major.
Turnock et al. (2007)
24 more

12 mentions without citation

The Potential of Secondary Metabolites from Plants as Drugs or Leads against Protozoan Neglected Diseases-Part III: In-Silico Molecular Docking Investigations.
Ogungbe et al. (2016)
11 more