› 1gz0

X-ray diffraction
2.5Å resolution

23S RIBOSOMAL RNA G2251 2'O-METHYLTRANSFERASE RLMB

Released:
DOI: 10.2210/pdb1gz0/pdb
PDB entry 1gz0 coloured by chain, front view.
PDB entry 1gz0 coloured by chain, side view.
PDB entry 1gz0 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The structure of the RlmB 23S rRNA methyltransferase reveals a new methyltransferase fold with a unique knot.
Michel G, Sauvé V, Larocque R, Li Y, Matte A, Cygler M
Structure 10 1303-15 (2002)
PMID: 12377117

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + guanosine(2251) in 23S rRNA = S-adenosyl-L-homocysteine + 2'-O-methylguanosine(2251) in 23S rRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159030 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
23S rRNA (guanosine-2'-O-)-methyltransferase RlmB Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 253 amino acids
Theoretical weight: 27.94 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P63177 (Residues: 1-243; Coverage: 100%)
Gene names: JW4138, b4180, rlmB, yjfH
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 64 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P21
Unit cell:
a: 87.478Å b: 132.288Å c: 90.71Å
α: 90° β: 96.4° γ: 90°
R-values:
R R work R free
0.231 0.231 0.279
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Structural and sequence motifs of protein (histone) methylation enzymes.
Cheng et al. (2005)
10 more

13 mentions without citation

Many paths to methyltransfer: a chronicle of convergence.
Schubert et al. (2003)
12 more